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Dr. Raul Almeida DVM, UNL Argentina Research Assistant Professor Primary Affiliation: UT AnSci Expertise: Microbial Pathogenesis Email: ralmeida@utk.edu

[ Preparation / Appointments / Publications / Awards / Activities / Collaborators ]

Professional Preparation:

Universidad Nacional del Litoral   DVM   1977   Veterinary Medicine
Iowa State University              MSc   1989 Veterinary Microbiology
Iowa State University              PhD   1991   Veterinary Microbiology

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Appointments:

Research Assistant Professor, Department of Animal Science, The University of Tennessee, 2001-present. Research Scientist, Department of Animal Science, The University of Tennessee, 1998-present. Post-Doctoral Research Associate, Department of Animal Science, The University of Tennessee, 1992-1998. Research Associate, Veterinary Medical Research Institute, Department of Veterinary Microbiology, Iowa State University, 1986-1991. Coordinator, Veterinary Diagnostic Unit, National Institute of Agricultural Technology, Rafaela Experimental Station, 1982-1985. Researcher in Charge, Veterinary Microbiology Laboratory, Veterinary Diagnostic Unit, National Institute of Agricultural Technology, Argentina, Rafaela Experimental Station, 1977-1982.

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Publications:

Patel, D., R. A. Almeida, J. R. Dunlap, and S. P. Oliver. 2009. Bovine lactoferrin serves as a molecular bridge for internalization of Streptococcus uberis into bovine mammary epithelial cells. Vet Microbiol. 137(3-4):297-301

Luther, D. A., R. A. Almeida and S. P. Oliver. 2008 Elucidation of the DNA sequence of Streptococcus uberis adhesion molecule gene (sua) and detection of sua in strains of Streptococcus uberis isolated from geographically diverse locations Vet. Microbiol.128:304-12.

Cesaris, L., B. E. Gillespie, V. Srinivasan, R. A. Almeida, A. Zecconi, and S. P. Oliver. 2007. Discriminating Between Strains of Escherichia coli Using Pulsed-Field Gel Electrophoresis and BOX-PCR. Foodborne Pathog. Dis. 4:473-80.

Almeida, R. A., D. Patel, G. M. Friton and S. P. Oliver. 2007. Intracellular killing of mastitis pathogens by penethamate hydriodide following internalization into mammary epithelial cells. J. Vet. Pharmacol. Therap. 30:151-156.

Dogan, B., S. Klaessing, M. Rishniw, R. A. Almeida, S. P. Oliver, K. Simpson, and Y. H. Schukken. 2006. Adherent and invasive Escherichia coli are associated with persistent bovine mastitis.  Vet.  Microbiol. 116: 270-82

Almeida R. A. and S. P. Oliver. 2006. Trafficking of Streptococcus uberis in bovine mammary epithelial cells. Microbial Pathogenesis 41:80-89.

Almeida, R. A., D. A, Luther, H. M. Park, and S. P. Oliver. 2006. Identification, isolation, and partial characterization of a novel Streptococcus uberis adhesion molecule (SUAM). Vet. Microbiol. 115: 183–191.

Batcha T., R. A. Almeida, J. R. Dunlap, S. P. Oliver.  2006. Streptococcus uberis internalizes and persists in bovine mammary epithelial cells. Microbial Pathogenesis 40: 279–285.

Oliver, S. P., B. M. Jayarao and R. A. Almeida. 2005.  Foodborne pathogens in milk and the
dairy farm environment: food safety and public health implications. Foodborne Pathog Dis. 2:115-29.

Hockett, M. E., R. A. Almeida, N. R. Rohrbach, S. P. Oliver, H. H. Dowlen, and F. N. Schrick. 2005. Effects of Induced Clinical Mastitis During Preovulation on Endocrine and Follicular Function. J. Dairy Sci. 88: 2422-2431.

Rambeaud M., R.A. Almeida, and S. P. Oliver. 2004. Growth of Streptococcus uberis in skim milk obtained from Holstein and Jersey dairy cows during different stages of lactation. J Vet Med B Infect Dis Vet Public Health. 51:143-145


Murinda, S.E,.  L. T. Nguyen, H. M. Nam, R.A. Almeida, S. J. Headrick and S. P. Oliver. 2004. Detection of sorbitol-negative and sorbitol positive Shiga toxin-producing Escherichia coli, Listeria monocytogenes, Campylobacter jejuni and Salmonella spp. in dairy environmental samples. Foodborne Pathogens and Disease. 1: 97-104

Rambeaud M., R.A. Almeida, G. M. Pighetti and S. P. Oliver.  2003. Dynamics of leukocytes and cytokines during experimentally induced Streptococcus uberis mastitis. Vet Immunol Immunopathol. 96:193-205

Almeida, R. A., D.A. Luther, R. Nair and S. P. Oliver 2003. Binding of host glycosaminoglycans and milk proteins: possible role in the pathogenesis of Streptococcus uberis mastitis. Vet Microbiol. 2003 94:131-41

Murinda, S. E., L. T. Nguyen, S. J. Ivey, R. A. Almeida, F. A. Draughon and S. P. Oliver. 2002. Prevalence and molecular characterization of Escherichia coli O157: H7 in bulk tank milk and fecal samples from cull cows: a 12-month survey of dairy farms in east Tennessee. J Food Prot. 65:752-759.

Murinda, S. E., L. T. Nguyen, S. J. Ivey, R. A. Almeida and S. P. Oliver. 2002. Novel single-tube agar-based test for motility enhancement and immunocapture of Escherichia coli 0157:H7 by H7 flagellar antigen-specific antibodies. J. Clin. Microbiol. 40:4685-4690.

Murinda, S. E., L. T. Nguyen, S. J. Ivey, B. E. Gillespie, R. A. Almeida and S. P. Oliver. 2002 Molecular characterization of Salmonella spp. isolated from bulk tank milk and cull dairy cow fecal samples. J Food Prot. 65:1100-1105.

Murinda, S. E., L. T. Nguyen, S. J. Ivey, B. E. Gillespie, R. A. Almeida and S. P. Oliver. 2002 Molecular characterization of Salmonella spp. isolated from bulk tank milk and cull dairy cow fecal samples. J Food Prot. 65:1100-1105.


Park, H. M., R. A. Almeida, and  S. P. Oliver, 2002 Identification of lactoferrin-binding proteins in Streptococcus dysgalactiae subsp. dysgalactiae and Streptococcus agalactiae isolated from cows with mastitis. FEMS Microbiol.  Lett.  207:87-90

Murinda, S. E., L. T. Nguyen, S. J. Ivey, B. E. Gillespie, R. A. Almeida and S. P. Oliver. 2002. Prevalence and molecular characterization of Escherichia coli 0157:H7 in bulk tank milk and fecal samples of cull dairy cows: a 12 months survey of dairy farms in East Tennessee. J. Food Protect.  65:752-759

Almeida, R. A., and S. P.  Oliver. 2001. Role of collagen in adherence of Streptococcus uberis to bovine mammary epithelial cells. J. Vet. Med.  B. 48: 759-763

Almeida, R. A., and S. P. Oliver, 2001. Interaccion of coagulase-negative Staphylococcus species with bovine mammary epithelial cells. Microbial Pathogenesis. 31: 205-212

Calvinho, L. F., R. A. Almeida, and S. P. Oliver. 2001. Influence of bacterial factors on proliferation of bovine mammary epithelial cells. Rev. Arg. Microbiol. 33: 28-35

Oliver, S. P. B. E. Gillespie, M. J. Lewis, S. J. Ivey, R. A. Almeida, D. A. Luther, D. L. Johnson, K. C. Lamar, H. D. Moorehead, and H. H. Dowlen. 2001. Efficacy of a new pre-milking teat disinfectant containing a phenolic combination for the prevention of mastitis. J. Dairy Sci. 84: 1545-1549

Park, H. M,  R. A. Almeida,  D. A. Luther, and S. P. Oliver. 2001. Binding of bovine lactoferrin to Streptococcus dysgalactiae subsp. dysgalactiae isolated from cows with mastitis. FEMS Microbiol. Lett. 208:35-39

Dopfer, D., H. Nederbragt, R. Almeida, and W. Gaastra. 2001.Studies about the mechanism of internalization by mammary epithelial cell of Escherichia coli isolated from persistent bovine mastiits. Vet. Microbiol. 80:285-296.

Almeida R. A., L. F. Calvinho and S. P. Oliver. 2000. Influence of protein kinase inhibitors on Streptococcus uberis internalization into bovine mammary epithelial cells. Microbial Pathogenesis 28:9-16.

Dopfer, D., R. A. Almeida, T. J. G. M. Lam, H. Nederbragt, S. P. Oliver and W. Gaastra. 2000 Adhesion and invasion of Escherichia coli from recurrent clinical cases of bovine mastitis. J. Vet. Microbiol. 12; 74(4): 331-43.

Fang, W., R. A. Almeida, and S. P. Oliver. 2000. Effects of lactoferrin and milk on adherence of Streptococcus uberis to bovine mammary epithelial cells. Am. J. Vet. Res.  61:275-279.

Oliver, S. P., M. J. Lewis, B. E. Gillespie, S. Ivey, L. Coleman, R. A. Almeida, W. Fang and K. Lamar. 2000. Evaluation of a post-milking teat disinfectant containing phenol for the prevention of bovine mastitis during lactation. J. Food Prot. 62: 11: 1354-1357.

R. A. Almeida, W. Fang, and S. P. Oliver. 1999 Adherence and internalization of Streptococcus uberis to bovine mammary epithelial cells are mediated by host cell proteoglycans. FEMS Microbiol. Lett. 177:313-317

R. A. Almeida,  D.A. Luther, S. P. Oliver. 1999. Incubation of Streptococcus uberis with extracellular matrix proteins enhances adherence to and internalization into bovine mammary epithelial cells. FEMS Microbiol. Lett. 178:81-85.
22. Fang, W., D. A. Luther, R. A. Almeida and S. P. Oliver. 1998. Decreased growth of Streptococcus uberis in milk from mammary glands challenged with the same mastitis pathogen. J. Vet. Med. B. 45:539-549.

23. Oliver, S. P., R. A. Almeida and Calvinho L. F, 1998. Virulence factors of Streptococcus uberis isolated from cows with mastitis. J. Vet. Med. B 45:461-471.

24. Calvinho, L. F., R. A. Almeida and S. P. Oliver, 1998. Potential Virulence factors of Streptococcus dysgalactiae associated with bovine mastitis. Vet. Microbiol. 61, 91-110.

25. Oliver, S. P., R. A. Almeida and W. Fang. 1998. New horizons in mastitis control. In: Proc. Pan-American Congress on Mastitis Control and Milk Quality.  Mérida, Mexico.

26. Almeida, R. A., K. R. Matthews and S. P. Oliver. 1997. Eukaryotic and Prokaryotic Cell Functions Required for Invasion of Staphylococcus aureus into Bovine Mammary Epithelial Cells. J. Vet. Med. B. 44:139-145.
27. Almeida, R. A., D. A Luther, S. K. Kumar, A.  L. F. Calvinho, and S. P. Oliver. 1996.
Adherence of Streptococcus uberis to bovine mammary epithelial cells and extracellular matrix proteins. J. Med. Vet. B. 43:385-392.

Almeida, R. A., K. R. Matthews, E. Cifrian, A. J. Guidry, and S. P Oliver. 1996. Staphylococcus aureus invasion of bovine mammary epithelial cells. J. Dairy Sci. 79:121-126.

Calvinho, L.F., R. A. Almeida, and S.P. Oliver. 1996. Influence of Streptococcus dysgalactiae surface hydrophobicity on adherence to mammary epithelial cells and phagocytosis by mammary macrophages. J. Vet. Med. B. 43:257-266.

Almeida, R. A., K. R. Matthews, E. Cifrian, A. Guidry, and S. P. Oliver.1996. Internalization of Staphylococcus aureus by bovine mammary epithelial cells. J. Dairy Sci 73 (Suppl. 2):18-33.

Almeida, R. A. and S. P. Oliver, 1995. Phagocytosis of Streptococcus uberis by bovine mammary macrophages: Opsonizing effect of bovine antiserum. J. Vet. Med. B. 42:331-337.

Almeida, R. A. and S. P. Oliver. 1995. Invasion of bovine mammary epithelial cells by
      Streptococcus dysgalactiae. J. Dairy Sci. 78:1310-1317.

Almeida, R. A. and S. P. Oliver. 1995. Interaction of Streptococcus uberis with bovine mammary epithelial cells. J. Dairy Sci. 78:173.

Almeida, R. A. and R. F. Rosenbusch, 1994. Tracheobronchial clearance in calves infected with Mycoplasma dispar. J. Vet. Med. Series B. 41:473-482.

Matthews, K. R., R. A. Almeida, and S. P. Oliver, 1994. Bovine mammary epithelial cell invasion by Streptococcus uberis. Infect. Immun. 62:5641-5646.

Almeida, R. A. and S. P. Oliver. 1993. Growth cycle, capsule expression and characterization of the capsular material of selected strains of Streptococcus uberis.
      J. Vet. Med. Series B. 40:707-714.

Almeida, R. A. and S. P. Oliver. 1993. Antiphagocytic effect of the capsule of Streptococcus uberis. J. Vet. Med. Series B. 40:697-706.

Almeida, R. A., D. H. Luther, S. J. Kumar, J. D. Turner, and S. P. Oliver. 1993.Adherence of encapsulated and non-encapsulated strains of Streptococcus uberis to mammary epithelial cells and extracellular matrix proteins. J. Dairy Sci.76: S1, 259.

Almeida, R. A., J. D. Turner, and S. P. Oliver. 1993. Penetration of bovine mammary epithelial cells by encapsulated and non-encapsulated strains of Streptococcus uberis. J. Dairy Sci. 76:S1, 259.

D. A. Luther, B. M. Jarayao, R. A. Almeida, and S. P. Oliver. 1993. Functional, antigenic and genetic analysis of IgG and BSA-binding proteins of Streptococcus dysgalactiae of bovine origin. J. Dairy Sci. 76:S1, 260.

Almeida, R. A., and S. P. Oliver. 1992. Characterization of the growth curve and expression of capsular material by two strains of Streptococcus uberis. J. Dairy. Sci. 75: S1 258.

Almeida, R. A., and R. F. Rosenbusch. 1992. Interaction of Mycoplasma dispar with bovine alveolar macrophages. Infect. Immun. 60:2914-2919.

Almeida, R. A., and R. F. Rosenbusch. 1991. Capsule-like material of Mycoplasma dispar induces by in vitro coculture with bovine cells is antigenically related to similar structures expressed in vivo. Infect. Immun. 59:3119-3125.

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Honors and Awards/Grants and Contracts:

Honors and Awards: Honor Membership of The Society of Phi Zeta Gamma Chapter, Honor Society of Veterinary Medicine,  Iowa State University, November, 1989. Invited paper on pathogenesis of streptococcal bovine mastitis.  Chonnam National University. Kwangju, South Korea, 2002. Invited paper  on virulence factors of Streptococcus uberis and their relationship with the pathogenesis of bovine mastitis". Annual Meeting of the Korean Association for Buiatrics, South Korea, 2002. Invited paper on Identification & Validation of critical control points during the harvesting of milk. International workshop on the Safety of Foods of animal Origin. Hanghzou, China , 2002. Invited speaker on interaction between host cells and bacterial pathogens, Post-graduate program in Veterinary Medicine, UNL, College of Veterinary Medicine, Santa Fe, Argentina, 2000. Invited paper on pathogenesis of bovine mastitis. ALMAST.  Sociedad Rural de Villa Maria, Argentina. Invited paper on application of HACCP to the control of mastitis, Veterinary College of Buenos Aires Province, Mar del Plata, 1999, Argentina. Invited paper on etiology of bovine infectious keratoconjunctivitis, State College of Veterinary Medicine, Cordoba,  Argentina, 1984.  Invited paper on etiology and pathology of bovine calf pneumonia. Argentine Society for Veterinary Medicine, Buenos Aires, Argentina, 1982. Invited paper on etiology of bovine mastitis. Argentine Society for Microbiology, 1980.

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Profile last updated on: 11/10/2009 2:11:45 PM